DNA Polymerase I Large (Klenow) Fragment from Promega

Product Specs
ItemDNA Polymerase I Large (Klenow) Fragment
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberM2201
Concentration150u 5–10u/μl
Quantity150u

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme that incorporates 10nmol of total deoxyribonucleotides into TCA-insoluble material in 30 minutes at 37°C. The reaction conditions are: 67mM potassium phosphate (pH 7.4 at 25°C), 6.7mM MgCl2, 1mM DTT, 50μg/ml activated calf thymus DNA and 33μM each of dATP, dCTP, dGTP and dTTP (a mix of unlabeled and [3H]dTTP).

Comments:
M2201

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Colorectal Cancer Cells Enter a Diapause-like DTP State to Survive ChemotherapyCellJanuary 7, 2021Rehman SK, Haynes J, Collignon E, Brown KR, Wang Y, Nixon AML, Bruce JP, Wintersinger JA, Singh Mer A, Lo EBL, et al.stain (DNA Polymerase 1 Large (Klenow) Fragment from Promega; dATP, dCTP, dGTP from Promega, Cat # M2201; Bio-11-dUTP from Cedarlane) (Wijsman et al., 1993), Ki-67 antibody (Novus Biologicals, Cat # NB110–90592,

(2) Commensal Neisseria kill Neisseria gonorrhoeae through a DNA-dependent mechanism in miceCell Host & MicrobeAugust 1, 2019Won Jong Kim, Dustin Higashi, Maira Goytia, Maria A. Rendón, Michelle Pilligua-Lucas, Matthew Bronnimann, Jeanine A. McLean, Joseph Duncan, David Trees, Ann E. Jerse, et al.M0226L Phusion Polymerase,Thermo Fisher,Cat# F531L DNA Polymerase I Klenow Fragment,Promega,Cat# M2201 Bacterial strains (Table S1),Bacterial strains (Table S1),Bacterial strains (Table S1) E. coli (DH5α),Lab

(3) Genetic variation affecting DNA methylation and the human imprinting disorder, Beckwith-Wiedemann syndromeClinical epigeneticsAugust 30, 2018Dagar V, Hutchison W, Muscat A, Krishnan A, Hoke D, Buckle A, Siswara P, Amor DJ, Mann J, Pinner J, et al.extension of the fill-in-oligo with the addition of 1 μL DNA Polymerase I (Large Klenow) fragment (Cat #M2201, Promega), 1 mM each of dTTP, dATP and dGTP (Cat #BIO-39025, Bioline) and 50 μM 5-aza-dCTP (Cat #NU-1118S,

(4) The caspase-3-p120-RasGAP module generates a NF-κB repressor in response to cellular stressJournal of cell scienceSeptember 15, 2015Khalil H, Loukili N, Regamey A, Cuesta-Marban A, Santori E, Huber M, Widmann C.annealed probe was labeled with [32P]dCTP (5 µCi) using Klenow DNA polymerase (2 U/µl; Promega cat. no. M2201). 4 ng of the labeled probe and 10 µg of nuclear extracts were mixed in an equal volume of 2× binding

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